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soil. It is also used to assess whether a soil is appropriate for testing under such guidelines like the OECD 307 Guideline ‘Aerobic and Anaerobic Transformation in Soil’ and the EPA Guideline OCSPP 835.4100 ‘Aerobic Soil Metabolism’. It is used for the same purpose for soil toxicity testing in the OECD 216/217 Soil Microorganisms: Nitrogen and Carbon Transformation Test Guidelines. These guidelines state that a well‐established microbial population in soil and one that is appropriate for testing equals 1% of the soil’s organic carbon content. This value is important for testing to ensure the soil is representative of soils in nature and can provide accurate degradation rates in soil metabolism studies and meaningful toxicity values in the nitrogen and carbon transformation (a.k.a., soil microflora) testing.